Genemed Synthesis Inc.
Genemed Synthesis Inc.
Excellence by Design, Dedication, and Experience
Tuesday May 23, 2017
Sign In     My Cart    My Account    Corporate    Distributors    Customer Service    Info@genemedsyn.com    Toll Free (800) 344-5337
Search
Browse By
New Products
Special Promotions
Request A Catalog
Request Quote
Custom Peptide Synthesis & Antibodies
Recently Viewed Items
Join Mailing Lists
    

 

 

Home PageOnline Product Catalog > Custom Antibodies > Custom Peptide Synthesis & Antibodies

Genemed Synthesis Inc. (GSI) has been providing custom peptide and antibody services for the last 20 years. Our products and services have been received and appreciated by Scientists from all over the world and 1000s of publications. We strive hard to provide best possible service and competitive prices.
  • GSI has >~20 years of track record.
  • GSI offers the most comprehensive service not only to find antigenic peptides but also to learn a lot more about the protein structure, intracellular location, and potential functions. There is no charge for this service.
  • GSI is one of the most cited companies for custom peptide and antibodies. GSI has been cited in 1000s of top-ranked journals such as Nature, Science, Cell, JBC, PNAS, etc.
  • GSI offers the highest level of quality and service for very competitive prices. GSI will meet or exceed a quote for identical service from any other US company.

Custom Peptide (non-modified) Synthesis, Conjugation, and Anti-Peptide Antibodies


Summary of Services Included in Various Packages

Items/
Services
Std Package
Cat# CPAR-2
Option to add ELISA or Affinity Purification
Cat# CPAR-2A
FreeProtein sequence
& antigenicity analyses
Peptides Synthesis (up to 20-aa)
(70-85% pure, mass spec, hplc)
10-20 mg total
10-20 mg total
Supply of free peptide
5 mg
~ 5 mg
Peptide Conjugation
(couple ~ 5-10 mg to KLH etc)
Animals (2 rabbits or other animals)
& 70-days immunization program
Pre-immune bleeds (2-5 ml/animal)
Production Bleeds
(15-25 mls/animal) total 80-100 mls
ELISA Testing of 1st bleeds (optional)
Affinity Purification (includes coupling of ~ 5 mg peptide to Sepharose, purification of 1 bleed, ELISA testing of bleeds and supply of the affinity column)
 
Total Duration
12-14 weeks
14-16 weeks

Peptide Synthesis and Antibody Ordering Information

Detailed protocol for custom Polyclonal Antibodies 
 
All projects include 2 rabbits but we can substitute them with 2 G. Pig/Hamsters/or Chicken at no extra charge. Goat and sheep will incur additional charges. We can modify the std immunization and bleed protocols and include more animals if requested. Other animals, not included above, can be provided as well. Please contact GSI for current prices and availability. Std 70 days protocol include up to 5 injections (i.m. and s.c. at multiple sites at 15 days intervals); 1 pre-immune bleed (~2 ml per animal), 2 immune bleeds (std. bleeds at weeks 7 and 9; ~15-20 mls per rabbit). All projects may be extended on a monthly basis, for a nominal fee, to collect more antiserum (2 bleeds per month). Please inform us prior to the conclusion of std protocol if you wish to extend the projects.
 
Affinity purification. This package typically includes: Coupling of peptide (up to 10 mg to Sepharose), purification of any 1 bleed of ~ 15 ml or a pool of antiserum up to 15 ml; ELISA testing of both unpurified and affinity pure antibodies, and supply of affinity column. We also monitor the antibody activity in the unbound fraction to make sure antibodies are not overloaded and wasted. Ideally, we need approx. ~10 mg of antigen or peptide for making the affinity matrix. This package(Cat # CPAF-1; $595)can also be ordered separately. Note: Affinity purification is normally scheduled on 2nd bleed (pool of sera from both rabbits) unless antibody titer is poor in the first and 2nd bleed. However, user may elect to delay affinity purification or use just 1 rabbit serum. We must be informed prior to the second bleed.
 
ELISA. GSI uses proprietary techniques and buffer solutions to coat free peptides on ELISA plates. Therefore antibodies to the carrier protein are not detected. We test individual preimmune and immune serum at 4 dilutions (1:100, 1:1K, and 1:10K, 1:100K). A detailed ELISA report is provided with every test regarding antibody titer of each animal. Antibody titer reported in our ELISA report refers only to anti-peptide antibodies.


Genemed Synthesis Inc.| 6203 Woodlake Center Bldg#2 | San Antonio, Texas 78244 USA
Toll Free: (800)-344-5337 | 210-745-5988 | Fax: 210-569-6374| 210-745-5992 | Web: www.genemedsyn.com | Contact GSI
 Return Policy | PRIVACY STATEMENT
Copyright ©2015.  All rights reserved.