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Genemed Synthesis Inc. (GSI) has been providing custom peptide and antibody services for the last 20 years. Our products and services have been received and appreciated by Scientists from all over the world and 1000s of publications. We strive hard to provide best possible service and competitive prices.
- GSI has >~20 years of track record.
- GSI offers the most comprehensive service not only to find antigenic peptides but also to learn a lot more about the protein structure, intracellular location, and potential functions. There is no charge for this service.
- GSI is one of the most cited companies for custom peptide and antibodies. GSI has been cited in 1000s of top-ranked journals such as Nature, Science, Cell, JBC, PNAS, etc.
- GSI offers the highest level of quality and service for very competitive prices. GSI will meet or exceed a quote for identical service from any other US company.
Custom Peptide (non-modified) Synthesis, Conjugation, and Anti-Peptide Antibodies
Summary of Services Included in Various Packages
Peptide Synthesis and Antibody Ordering Information
Detailed protocol for custom Polyclonal Antibodies
All projects include 2 rabbits but we can substitute them with 2 G. Pig/Hamsters/or Chicken at no extra charge. Goat and sheep will incur additional charges. We can modify the std immunization and bleed protocols and include more animals if requested. Other animals, not included above, can be provided as well. Please contact GSI for current prices and availability. Std 70 days protocol include up to 5 injections (i.m. and s.c. at multiple sites at 15 days intervals); 1 pre-immune bleed (~2 ml per animal), 2 immune bleeds (std. bleeds at weeks 7 and 9; ~15-20 mls per rabbit). All projects may be extended on a monthly basis, for a nominal fee, to collect more antiserum (2 bleeds per month). Please inform us prior to the conclusion of std protocol if you wish to extend the projects.
Affinity purification. This package typically includes: Coupling of peptide (up to 10 mg to Sepharose), purification of any 1 bleed of ~ 15 ml or a pool of antiserum up to 15 ml; ELISA testing of both unpurified and affinity pure antibodies, and supply of affinity column. We also monitor the antibody activity in the unbound fraction to make sure antibodies are not overloaded and wasted. Ideally, we need approx. ~10 mg of antigen or peptide for making the affinity matrix. This package(Cat # CPAF-1; $595)can also be ordered separately. Note: Affinity purification is normally scheduled on 2nd bleed (pool of sera from both rabbits) unless antibody titer is poor in the first and 2nd bleed. However, user may elect to delay affinity purification or use just 1 rabbit serum. We must be informed prior to the second bleed.
ELISA. GSI uses proprietary techniques and buffer solutions to coat free peptides on ELISA plates. Therefore antibodies to the carrier protein are not detected. We test individual preimmune and immune serum at 4 dilutions (1:100, 1:1K, and 1:10K, 1:100K). A detailed ELISA report is provided with every test regarding antibody titer of each animal. Antibody titer reported in our ELISA report refers only to anti-peptide antibodies.
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